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Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi
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Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

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Coinfection with BHV-1 modulates cell adhesion and invasion by P. multocida and Mannheimia (Pasteurella) haemolytica

M Galdiero1, M G Pisciotta, A Marinelli

  • 1Dipartimento di Medicina Sperimentale, Facoltd di Medicina e Chirurgia, Seconda Università di Napoli.

The New Microbiologica
|November 20, 2002
PubMed

Insights

Bovine herpesvirus type 1 (BHV-1) infection increases MDBK cell susceptibility to Pasteurella multocida and Mannheimia haemolytica. Viral infection enhances bacterial adherence and invasion, particularly for M. haemolytica.

Area of Science:

  • Veterinary Virology
  • Bacterial Pathogenesis
  • Cellular Microbiology

Background:

  • Viruses are known to exacerbate bacterial respiratory tract infections.
  • Understanding virus-bacteria interactions is crucial for managing animal diseases.

Purpose of the Study:

  • To investigate the impact of Bovine herpesvirus type 1 (BHV-1) on the adherence and invasion of MDBK cells by Pasteurella multocida and Mannheimia haemolytica.
  • To determine how viral infection influences the susceptibility of host cells to bacterial pathogens.

Main Methods:

  • MDBK cells were infected with BHV-1 at varying pre-incubation times and virus concentrations.
  • Adherence and invasion assays were performed using P. multocida and M. haemolytica.
  • Cell susceptibility was quantified via bacterial counts post-infection.

Main Results:

  • BHV-1 infection significantly increased MDBK cell adherence for both P. multocida and M. haemolytica.
  • The degree of adherence enhancement correlated with virus pre-incubation time and concentration.
  • Uninfected MDBK cells were only invaded by P. multocida; BHV-1 infection enabled M. haemolytica invasion.
  • Viral infection generally promoted bacterial invasion of MDBK cells.

Conclusions:

  • BHV-1 infection enhances MDBK cell susceptibility to adherence and invasion by P. multocida and M. haemolytica.
  • This viral-host interaction may play a significant role in the pathogenesis of bovine respiratory disease.
  • Targeting viral co-infections could be a strategy to mitigate bacterial respiratory infections in cattle.

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