Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular analysis of SNAP-25 function in exocytosis.

Margaret E Graham1, Philip Washbourne, Michael C Wilson

  • 1The Physiological Laboratory, University of Liverpool, Liverpool, L69 3BX, United Kingdom.

Annals of the New York Academy of Sciences
|November 20, 2002
PubMed
Summary

Researchers investigated the molecular mechanisms of membrane fusion in exocytosis using advanced techniques. They found that specific modifications to SNARE proteins, particularly SNAP-25

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Salts on the Aggregation and Strength of Protein-Based Underwater Adhesives.

ACS omega·2025
Same author

Transition of Structurally Distinct Amyloids in the Degradation of Protein Materials.

The journal of physical chemistry. B·2025
Same author

Probing Molecular Interactions between Barnacle Peptides and Polymers <i>In Situ</i> to Understand Bioadhesion.

The journal of physical chemistry letters·2025
Same author

Can we model autism using zebrafish?

Development, growth & differentiation·2023
Same author

Role of protein aggregate structure on the strength and underwater performance of barnacle-inspired adhesives.

Soft matter·2023
Same author

Unraveling Presenilin 2 Functions in a Knockout Zebrafish Line to Shed Light into Alzheimer's Disease Pathogenesis.

Cells·2023

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Neuroscience

Background:

  • SNARE proteins are essential for intracellular membrane fusion, particularly in exocytosis.
  • Understanding the precise molecular roles of SNAREs and their regulators is crucial for deciphering physiological membrane fusion.
  • Existing methods lack the resolution to dissect the kinetics of exocytosis at the molecular level.

Purpose of the Study:

  • To dissect the molecular roles of SNARE proteins and their regulators in physiological membrane fusion during exocytosis.
  • To investigate the function of SNAP-25B, focusing on palmitoylated cysteines and conserved residues in the SNARE complex.
  • To reveal novel roles of proteins in the late stages of exocytosis.

Main Methods:

  • Developed approaches to manipulate protein expression in model secretory cells (PC12 and adrenal chromaffin cells).

Related Experiment Videos

  • Assayed exocytosis at high-time resolution using carbon-fiber amperometry to measure kinetics of single secretory granule release events.
  • Utilized a SNAP-25B mutant resistant to Clostridial neurotoxin BoNT/E to analyze mutations in the absence of endogenous protein.

    • Main Results:

    • Manipulation of proteins involved in the exocytotic machinery led to detectable changes in exocytosis kinetics, revealing novel roles in late stages.
    • A SNAP-25 mutant resistant to BoNT/E successfully reconstituted exocytosis in treated cells.
    • Analysis of the SNAP-25 mutant suggests an important role for palmitoylated cysteines in triggered exocytosis, but not for 0 layer glutamines.

    Conclusions:

    • The study provides novel insights into the molecular mechanisms governing the kinetics of exocytosis.
    • Palmitoylated cysteines of SNAP-25 play a significant role in triggered exocytosis.
    • The conserved 0 layer glutamines of the SNARE complex appear less critical for triggered exocytosis compared to SNAP-25 cysteines.