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Related Experiment Videos

The complement factor 5a receptor gene maps to murine chromosome 7.

D M Shubitowski1, M Wills-Karp, S L Ewart

  • 1Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA.

Cytogenetic and Genome Research
|November 20, 2002
PubMed
Summary

Complement factor 5a (C5a) and its receptor C5r1 are implicated in asthma. This study mapped C5r1 to mouse chromosome 7, a region associated with airway hyperresponsiveness.

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Area of Science:

  • Immunology
  • Genetics
  • Pulmonology

Background:

  • Complement factor 5a (C5a) is a key mediator of inflammation and chemoattraction.
  • C5a was previously identified as a candidate gene (Abhr2) for allergen-induced bronchial hyperresponsiveness in a murine asthma model.
  • The role of C5a receptor 1 (C5r1) in this asthma model required its precise genomic mapping.

Purpose of the Study:

  • To determine the genomic map location of the C5a receptor 1 (C5r1) in mice.
  • To investigate the significance of C5r1 in a murine model of asthma.

Main Methods:

  • Analysis of genomic sequence surrounding murine C5r1 for polymorphisms.
  • Identification and genotyping of two variable microsatellites in 355 backcross mice.
  • Multipoint linkage analysis using 164 genome-wide markers.

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Main Results:

  • C5r1 was mapped to murine chromosome 7.
  • The map location of C5r1 was determined to be 3.9 cM from the top of the linkage group.
  • This location overlaps with a previously identified quantitative trait locus (Abhr3) for allergen-induced airway hyperresponsiveness.

Conclusions:

  • The genomic location of C5r1 on mouse chromosome 7 supports its potential role in asthma pathogenesis.
  • C5r1 is a positional candidate gene for Abhr3, contributing to airway hyperresponsiveness in this asthma model.