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Ultrastructural changes in the lungs induced by extracorporeal perfusion
Acta Morphologica Academiae Scientiarum Hungaricae
|January 1, 1975
Summary
Lung tissue changes occur during heart surgery with extracorporeal circulation. These alterations, particularly in capillaries and surfactant, may contribute to the postperfusion syndrome.
Area of Science:
- Cardiovascular Surgery
- Pulmonary Medicine
- Cell Biology
Background:
- Heart operations frequently involve extracorporeal circulation (ECC).
- Pulmonary complications, such as postperfusion syndrome, can arise after ECC.
- Understanding lung tissue response to ECC is crucial for patient outcomes.
Purpose of the Study:
- To investigate lung structural and functional changes during heart surgery with ECC.
- To identify specific cellular alterations in the pulmonary tissue.
- To explore the potential role of surfactant dysfunction in postperfusion syndrome.
Main Methods:
- Lung tissue samples were collected from 15 patients undergoing heart surgery with ECC.
- Samples were obtained pre-perfusion and post-perfusion after thoracotomy.
- Ruthenium red staining was used to examine surfactant properties.
Main Results:
- Pulmonary structural changes were observed even before the initiation of perfusion compared to controls.
- Significant alternations in capillary endothelial cells and basal membranes were noted.
- Ruthenium red staining indicated changes in surfactant reactions, suggesting surfactant affection.
Conclusions:
- Lung tissue exhibits pre-perfusion changes during cardiac surgery with ECC.
- Capillary endothelial cells and basal membranes are notably affected.
- Surfactant dysfunction is implicated as a potential factor in the development of postperfusion syndrome.