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Primer on medical genomics. Part IV: Expression proteomics
Animesh Pardanani1, Eric D Wieben, Thomas C Spelsberg
1Division of Hematology and Internal Medicine, Mayo Clinic, Rochester, Minn 55905, USA.
Mayo Clinic Proceedings
|November 21, 2002
Summary
Proteomics is the study of all proteins, their functions, and interactions. This review focuses on expression proteomics, which catalogs protein abundance using advanced technologies like mass spectrometry.
Area of Science:
- Proteomics: The comprehensive study of proteomes, encompassing protein abundance, modifications, functions, and interactions within biological systems.
Background:
- Proteins execute biological functions encoded by genes, necessitating proteomic studies after genome sequencing.
- Understanding the proteome is crucial for addressing fundamental biological questions.
Purpose of the Study:
- This article provides a review of expression proteomics.
- Expression proteomics focuses on cataloging the relative abundance of proteins.
Main Methods:
- Proteomics employs high-throughput technologies including 2D-PAGE, mass spectrometry, multidimensional liquid chromatography, and bioinformatics.
- Mass spectrometry is the preferred technology for protein identification, characterization, and sequencing.
- Availability of annotated genome databases facilitates proteomic analysis.
Main Results:
- Expression proteomics quantifies protein abundance on a global scale.
- Advanced technologies enable comprehensive protein analysis.
Conclusions:
- Proteomics is essential for biological research following genome sequencing.
- Expression proteomics is a key area for understanding cellular functions through protein abundance analysis.