Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence that C1s participates in the alternative complement pathway.

J E Volanakis, D R Schultz, R M Stroud

    International Archives of Allergy and Applied Immunology
    |January 1, 1976
    PubMed
    Summary

    The C1s subcomponent of complement C1 activates the alternative pathway under specific conditions. This finding suggests C1s plays a role in both classical and alternative complement pathway activation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Development of an integrative cross-omics approach for conceptual adverse outcome pathway network construction.

    Environment international·2026
    Same author

    High-pressure, high-temperature molecular doping of nanodiamond.

    Science advances·2019
    Same author

    The nature, origin and modification of insoluble organic matter in chondrites, the possibly interstellar source of Earth's C and N.

    Chemie der Erde : Beitrage zur chemischen Mineralogie, Petrographie und Geologie·2019
    Same author

    From amorphous to nanocrystalline: the effect of nanograins in an amorphous matrix on the thermal conductivity of hot-wire chemical-vapor deposited silicon films.

    Journal of physics. Condensed matter : an Institute of Physics journal·2017
    Same author

    Chemically exfoliating large sheets of phosphorene via choline chloride urea viscosity-tuning.

    Nanotechnology·2017
    Same author

    Use of the phosphorus detector as a confirmatory tool for measuring residues of shell chemical's triazine herbicide.

    Bulletin of environmental contamination and toxicology·2013

    Area of Science:

    • Immunology
    • Complement System Biology

    Background:

    • The complement system comprises classical and alternative pathways crucial for innate immunity.
    • C1s is a subcomponent of the classical pathway's C1 complex.
    • The precise role of C1s in the alternative pathway remains incompletely understood.

    Purpose of the Study:

    • To investigate the potential involvement of C1s in the alternative complement pathway.
    • To determine if C1s can initiate or modulate alternative pathway activation.

    Main Methods:

    • Experiments utilized sera genetically deficient in C2 or C4.
    • Hemolytic assays were performed to assess complement component consumption.
    • Immunoassays with anti-C1s antibodies and solid immunoabsorbents were employed to block or remove C1s activity.

    Main Results:

    • Purified C1s induced factor B conversion and C3/C5 consumption in C2- or C4-deficient sera.
    • Blocking C1s activity inhibited alternative pathway activation by zymosan and cobra venom factor.
    • C1s removal from C1r-deficient serum prevented alternative pathway activation by zymosan.

    Conclusions:

    • C1s can participate in and activate the alternative complement pathway under certain experimental conditions.
    • These findings suggest C1s may be a more general component in both classical and alternative pathway activation sequences.

    Related Experiment Videos