[Growth of three-dimensional complexes of living cells in the process of rotational cultivation]

Unlike the monolayer cultures, three-dimensional (multilayer) cultures of living cells make it possible to more closely imitate events occurring in native tissues. Rotating cultivators for three-dimensional cultures create analogous conditions of reduced gravity and, therefore, can be used in ground-based investigations of the biological effects of space flight. We have designed a disposable cultivation cell with diffused gas exchange and a device to rotate the cell at a controlled speed about the horizontal axis. Immortalized CH-fibroblasts were attached to a glass carrier pretreated with polylysine. Cultivation was performed for 2-3 days at 8 rot./min. A rapid growth of three-dimensional cell complexes was observed during the cultivation. The investigation demonstrated that multilayer cultures of immortalized CH-fibroblasts represent a convenient model for studies in cell biology and development of technologies of growing d-dimensional living cell complexes as a source of implants from autologic cells (including stem cells) thus resolving the problem of donator materials.