Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Globin gene switching in primates.

Robert M Johnson1, Deborah Gumucio, Morris Goodman

  • 1Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, MI 48201, USA. rmjohns@med.wayne.edu

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
|November 22, 2002
PubMed
Summary

Evolutionary analysis reveals conserved DNA motifs controlling globin gene transcription. These phylogenetic footprints dictate developmental expression timing, influencing globin switching in primates.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drosophila Col4a1 Glycine mutations highlight allelic heterogeneity and mechanistic pleiotropy.

Matrix biology : journal of the International Society for Matrix Biology·2025
Same author

Strategic re-engineering of antibiotics.

Nature reviews bioengineering·2025
Same author

Phosphorus Fluoride Exchange (PFEx) Click Chemistry: 2-Substituted-alkynyl-1-cyclotriphosphazene (SACP) Hubs for Diversity Oriented Clicking.

Advanced synthesis & catalysis·2025
Same author

Modular synthesis of functional libraries by accelerated SuFEx click chemistry.

Chemical science·2024
Same author

Diet-induced obesity and diabetes enhance mortality and reduce vaccine efficacy for SARS-CoV-2.

Journal of virology·2023
Same author

Shapeshifting bullvalene-linked vancomycin dimers as effective antibiotics against multidrug-resistant gram-positive bacteria.

Proceedings of the National Academy of Sciences of the United States of America·2023

Area of Science:

  • Genomics
  • Evolutionary Biology
  • Molecular Biology

Background:

  • The beta-globin cluster's gene transcription regulation is complex.
  • Understanding conserved regulatory elements is crucial for deciphering gene expression.
  • Evolutionary comparisons offer insights into functional DNA sequences.

Purpose of the Study:

  • To identify DNA sequences essential for beta-globin cluster gene transcription using evolutionary approaches.
  • To analyze the differential timing of gamma-globin gene expression in primates.
  • To investigate the role of conserved motifs in developmental stage-specific gene expression.

Main Methods:

  • Sequence alignments of non-coding regions from diverse species to identify conserved motifs (phylogenetic footprints).

Related Experiment Videos

  • Analysis of base changes near phylogenetic footprints to study differential gene timing.
  • Functional assays using transgenic mice with modified gamma-promoters.
  • Determination of globin gene and mRNA expression profiles during primate embryological development.

    • Main Results:

    • Conserved motifs (phylogenetic footprints) were identified as potential transcription factor binding sites.
    • Differential phylogenetic footprints bind distinct nuclear factors, influencing developmental timing.
    • DNA motifs near gamma-globin genes are sufficient to determine developmental expression stage.
    • Platyrrhine primates show variations in gamma 1 gene inactivation and an earlier gamma-beta switch compared to catarrhines.

    Conclusions:

    • Evolutionary conserved DNA motifs play a critical role in regulating beta-globin cluster gene transcription and developmental timing.
    • Specific DNA motifs near gamma-globin genes control the developmental stage of expression.
    • Primate evolution has led to diverse strategies for globin gene regulation, including gamma 1 gene inactivation and altered globin switching.