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Related Experiment Videos

Structure and function of human stratum corneum under deformation.

Jean-Luc Lévêque1, Philippe Hallégot, Jean Doucet

  • 1L'Oréal Recherche, Centre Charles-Zviak, Clichy, France. jlleveque@recherche.loreal.com

Dermatology (Basel, Switzerland)
|November 22, 2002
PubMed
Summary

Mechanical stress on the stratum corneum (SC) up to 20% elongation does not significantly impact skin barrier function. High strain (60%) in vitro detached SC structures but did not alter lipid organization.

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Area of Science:

  • Dermatology
  • Biophysics
  • Materials Science

Background:

  • The stratum corneum (SC) is crucial for skin barrier function.
  • The impact of mechanical stress on SC barrier properties remains debated.

Purpose of the Study:

  • To investigate the effects of controlled mechanical strain on human SC structure and barrier function.
  • To assess transepidermal water loss (TEWL) in vivo and SC structure in vitro under varying degrees of skin extension.

Main Methods:

  • In vivo TEWL measurements on human skin subjected to 0-20% strain using a Densi-score device.
  • In vitro structural analysis of SC using X-ray diffraction and transmission electron microscopy (TEM) at high strain (60%).

Main Results:

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  • In vivo TEWL remained unaffected by skin extension up to 20%.
  • In vitro TEM revealed detachment of lipid bilayers and corneosomes from corneocytes at 60% strain.
  • X-ray diffraction showed no changes in intercellular lipid supramolecular organization during stretching.

    • Conclusions:

    • Mechanical strain up to 20% elongation does not significantly compromise the human SC barrier function.
    • High strain levels can disrupt SC structural components in vitro, but the overall barrier integrity is maintained up to 20%.