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Lipids are co-eluted with immunoglobulins G during purification by recombinant streptococcal protein G affinity
Eirini Kitsiouli1, Marilena E Lekka, George Nakos
1Laboratoire de Biogenèse Membranaire CNRS-UMR 5544, Université Victor Segalen, Bordeaux 2, France. me00157@cc.uoi.gr
Journal of Immunological Methods
|November 26, 2002
Abstract:
The efficiency of recombinant streptococcal protein G (rec-spG) affinity column chromatography in purifying immunoglobulins G (IgG) from lipids has been studied, with particular reference to IgG fractions from bronchoalveolar lavage (BAL) fluid and serum samples from different sources. It was found that the IgG fractions purified by rec-spG affinity column chromatography also contained cholesterol and phospholipids.