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Does growth hormone treatment alter skeletal muscle mitochondrial respiration in rats?
C Peyreigne1, E Raynaud, C Fedou
1Département de Physiologie, Laboratoire de Physiologie des Interactions, UPRES EA701, Institut de Biologie, Faculté de Médecine, Montpellier, France. c.peyreigne@laposte.net
Objective:
Growth hormone (GH) has been shown to stimulate lipolysis and enhance lipid oxidation. We investigated whether GH could improve mitochondrial oxidative capacity.
Method:
Fourteen male Wistar rats received 14-day treatment with biosynthetic human GH (10 IU/kg/24 h) or placebo. Mitochondria were isolated from the total muscle of one hind limb of the rat. Mitochondrial oxygen consumption was measured in vitro using a Clark-type electrode with three substrates: palmitoyl-L-carnitine, pyruvate and succinate (+ rotenone).
Results:
Muscle mitochondrial yield was not significantly different in the GH-treated group from that in controls. Neither the basal nor ADP-stimulated respiratory state reached a significant difference between the 2 groups with palmitoyl-L-carnitine, pyruvate, and succinate.
Conclusion:
GH treatment did not improve the oxidative capacity of skeletal muscle mitochondria.