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Related Experiment Videos

Extraembryonic proteases regulate Nodal signalling during gastrulation.

Séverine Beck1, J Ann Le Good, Marcela Guzman

  • 1Developmental Biology Group, Swiss Institute for Experimental Cancer Research (ISREC), Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland.

Nature Cell Biology
|November 26, 2002
PubMed
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Proprotein convertases Spc1 and Spc4 are essential for Nodal signaling during mammalian embryonic development. These enzymes mature Nodal protein, enabling antero-posterior pattern formation and Cripto induction.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Embryonic development relies on precise inductive signaling pathways.
  • Nodal signaling is crucial for establishing antero-posterior polarity during gastrulation.
  • Cripto acts as a co-receptor downstream of Nodal, mediating its effects.

Purpose of the Study:

  • To investigate the role of proprotein convertases Spc1 and Spc4 in Nodal signaling.
  • To determine the mechanism by which Nodal induces Cripto expression.
  • To elucidate the contribution of Spc1 and Spc4 to embryonic patterning.

Main Methods:

  • Analysis of Spc1 and Spc4 expression in extraembryonic ectoderm.
  • In vivo studies using Spc1(-/-); Spc4(-/-) double mutant mice.

Related Experiment Videos

  • Embryo explant experiments with and without extraembryonic ectoderm.
  • Functional rescue experiments using recombinant mature Nodal.

    • Main Results:

    • Spc1 and Spc4 are expressed in extraembryonic ectoderm and are required for Nodal signaling.
    • These proteases stimulate Nodal maturation and are essential for Cripto induction.
    • Lack of Spc1/Spc4 impairs Nodal-mediated Cripto induction and also affects Bone Morphogenetic Protein 4 (BMP4) induction.

    Conclusions:

    • Spc1 and Spc4 are critical for Nodal maturation and subsequent signaling during mammalian gastrulation.
    • Nodal induces Cripto through both direct embryonic signaling and indirect induction of BMP4 in extraembryonic tissues.
    • Spc1 and Spc4 regulate both Nodal maturation and BMP4 induction, highlighting their central role in embryonic patterning.