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Related Experiment Videos

Denitrifying activity measurements using an anoxic titration (pHstat) bioassay.

K M Foxon1, C J Brouckaert, A Rozzi

  • 1Pollution Research Group, School of Chemical Engineering, University of Natal, Durban, South Africa. foxonk@nu.ac.za

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|November 27, 2002
PubMed
Summary

A new titrimetric method accurately measures denitrifying activity in activated sludge. This approach uses an iterative algorithm to determine nitrate uptake rate (NUR) from pH data, improving wastewater treatment insights.

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Area of Science:

  • Environmental Science
  • Microbiology
  • Chemical Engineering

Background:

  • Denitrification is crucial for removing nitrogen from wastewater.
  • Accurate measurement of denitrifying activity is essential for optimizing activated sludge systems.
  • Existing methods for measuring denitrifying activity can be complex or indirect.

Purpose of the Study:

  • To investigate an anoxic titrimetric test for measuring denitrifying activity.
  • To develop a method for extracting nitrate uptake rate (NUR) from titration data.
  • To assess the reliability of the titrimetric method in activated sludge systems.

Main Methods:

  • An anoxic titrimetric test was conducted in batch denitrification experiments.
  • An automatic pH-stat acid dosing system was used to maintain pH set-points.

Related Experiment Videos

  • An iterative algorithm was implemented to analyze titration data and calculate NUR.
  • The simulation software package AQUASIM was used for data analysis.

    • Main Results:

    • The titrimetric method successfully measured denitrifying activity.
    • The iterative algorithm effectively extracted NUR data, accounting for by-product effects.
    • The method demonstrated potential for accurate assessment of microbial nitrogen removal.

    Conclusions:

    • The anoxic titrimetric test is a viable method for quantifying denitrifying activity.
    • This method provides a more direct and potentially more accurate measure of NUR.
    • The findings contribute to improved monitoring and control of biological nitrogen removal in wastewater treatment.