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An improved method for rapid ABO genotyping
1DNA Laboratory, Institute of Criminalistics, Police of Czech Republic, POB 62/KUP, Strojnická 27, 170 82 Prague 7, Czech Republic. danvanek@login.cz
Sbornik Lekarsky
|November 27, 2002
Abstract:
A method for ABO genotyping originally designed by Lee and Chang [3] and further developed by Akane et al. [7] has been even more simplified and improved. We obtained a rapid, robust, sensitive and low cost method for detection of sequence polymorphism of ABO glycosyltransferase gene by changing the MaeII restriction enzyme for its isoschizomer TaiI and by optimization of the condition during digestion and electrophoretic separation.