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[The SRM12/ADA1 gene acts as a cis-active factor when inserted into recombinant plasmids and makes them more stable

I A Chekhuta¹, I P Arman, A B Devin

¹Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia.

|November 27, 2002

Abstract:

A DNA fragment containing the SRM12/ADA1 gene sequence inserted into a recombinant circular plasmid improves its maintenance in budding yeast (Saccharomyces cerevisiae) cells. Plasmid stabilization caused by the integrated SRM12 sequence does not require the SRM12 function complementing the srm12 mutation and depends on the orientation of the inserted fragment in the vector. This stabilization is mainly due to a decrease in spontaneous plasmid underreplication/copy loss rather than an increase in the fidelity of mitotic plasmid segregation.

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