Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Completely isolated molluscan neurons. An ultrastructural study.

L S Bocharova, M A Kostenko, B N Veprintsev

    Brain Research
    |January 16, 1976
    PubMed
    Summary

    Isolated mollusc neurons maintain ultrastructure and metabolic activity, with membrane changes preceding electrical property alterations. This research aids understanding of neuronal cell isolation techniques.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Cyclic structural changes in endoplasmic reticulum and Golgi apparatus in the hippocampal neurons of ground squirrels during hibernation].

    Tsitologiia·2011
    Same author

    [Cryopreserved neurons of a mollusc are capable of morphological differentiation in culture in vitro].

    Biofizika·2004
    Same author

    [Proliferation and morphological differentiation of neurblastoma cells in cultured under the effect of avermectins].

    Morfologiia (Saint Petersburg, Russia)·2002
    Same author

    Methods and methodological approaches to studies of isolated neurons of brain from adult animals (Lymnaea stagnalis) in tissue culture.

    Neuroscience and behavioral physiology·1999
    Same author

    [Methodical and methodological approaches to the study of the isolated brain neurons from an adult animal (Lymnaea stagnalis) in tissue culture].

    Morfologiia (Saint Petersburg, Russia)·1998
    Same author

    Acridine orange as an indicator of the cytoplasmic ribosome state.

    Cytometry·1997

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Molluscan Physiology

    Background:

    • Studying isolated neurons is crucial for understanding cellular mechanisms.
    • Previous methods may have compromised neuronal integrity.

    Purpose of the Study:

    • To evaluate the ultrastructural and metabolic integrity of isolated mollusc neurons.
    • To compare isolated neurons with intact ones.
    • To investigate the relationship between ultrastructural changes and electrical properties.

    Main Methods:

    • Fermentative digestion and mechanical treatment for cell isolation.
    • Ultrastructural analysis using electron microscopy.
    • Measurement of RNA incorporation and membrane electrical properties.

    Related Experiment Videos

    Main Results:

    • Isolated neurons (Lymnaea, Helix) showed no ultrastructural differences from intact cells.
    • Metabolic reserves were sufficient, with significant RNA incorporation.
    • Neuronal damage (cytoplasmic vacuolization) occurred during pipetting, preceding electrical changes.
    • Neuron surface area doubled due to membrane infoldings, affecting membrane resistance.
    • Ultrastructural similarities to axo-somatic synapses were observed on isolated neuron surfaces.

    Conclusions:

    • Fermentative digestion and mechanical isolation preserve neuronal ultrastructure and metabolic function.
    • Ultrastructural integrity is a prerequisite for accurate electrophysiological measurements.
    • Isolated neurons offer a viable model for studying neuronal properties, with careful handling minimizing damage.