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Updated: Jul 30, 2026

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Published on: May 1, 2019
[Deacetylation of chitin under homogeneity]
S V Nemtsev1, A I Gamzazade, S V Rogozhin
1Bioinzheneriya Center, Russian Academy of Sciences, pr. Shestidesyatiletiya Oktyabrya 7-1, Moscow, 117312 Russia. varlamov@biengi.ac.ru
Abstract:
Chitin isolated enzymatically from Antarctic krill shells was dissolved in aqueous NaOH by freezing and thawing to create homogenous conditions. Deacetylation was performed at room temperature or heating. The degree of deacetylation, molecular weight, and dynamic viscosity of solutions were estimated in chitosan samples. Deacetylation of chitin under homogenous conditions was optimized. Chitosans with molecular weights of 180-220 and 250-300 kDa were obtained from the chitins of Antarctic krill and Northern shrimps, respectively.

