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Updated: Jul 29, 2026
Biochemical Measurement of Neonatal Hypoxia
Published on: August 24, 2011
Optimization and validation of an analytical procedure by high-performance liquid chromatography for the
A Osorio-Pérez1, M Salazar-Cavazos, A Piñeyro-López
1Department of Pharmacology and Toxicology, Faculty of Medicine, U.A.N.L., P.O. Box 2316, Suc. Tecnológico, 64841, Nuevo Leon, Monterrey, Mexico
Abstract:
Peroxisomicine A1 is a potential antineoplastic substance extracted from plants of the genus Karwinskia. An RP-HPLC-DAD method was developed and validated for the separation and quantification of four isomers of this compound. These isomers coelute in the preparative procedure and are present at a proportion ranging between 3 and 5% in the peroxisomicine A1 purified in the laboratory. The desirability coefficient of the method described here was enhanced 140% with respect to the previously reported method.
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