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Related Experiment Videos

Inhibitory effect of reserpine on dopamine transporter function.

Ryan R Metzger1, Jeffrey M Brown, Verónica Sandoval

  • 1Interdepartmental Program in Neuroscience, University of Utah, Salt Lake City 84112, USA.

European Journal of Pharmacology
|November 27, 2002
PubMed
Summary

Reserpine potently inhibits synaptosomal dopamine uptake and decreases dopamine transporter Vmax in rats. This effect appears dissociated from vesicular monoamine transporter-2 inhibition, suggesting an indirect mechanism.

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Area of Science:

  • Neuroscience
  • Pharmacology

Background:

  • Reserpine is known to affect monoamine neurotransmission.
  • Previous studies suggest reserpine may disrupt dopamine transporter (DAT) activity.

Purpose of the Study:

  • To investigate the effect of reserpine on dopamine transporter function.
  • To elucidate the mechanism by which reserpine influences dopamine uptake.

Main Methods:

  • Inhibition assays using synaptosomal [3H]dopamine uptake.
  • Ex vivo assessment of Vmax in reserpine-treated rats.
  • Experiments with vesicular monoamine transporter-2 (VMAT-2) knockout mice.
  • In vitro studies using cells expressing the human dopamine transporter.

Main Results:

Related Experiment Videos

  • Reserpine potently inhibits synaptosomal [3H]dopamine uptake.
  • Reserpine administration decreased the Vmax of synaptosomal dopamine transport ex vivo.
  • The observed decrease in Vmax was partially dissociated from VMAT-2 inhibition.
  • Reserpine did not inhibit dopamine uptake in cells expressing the human dopamine transporter.

    • Conclusions:

    • Reserpine may transiently alter dopamine transporter function.
    • The mechanism appears to be noncompetitive and indirect.
    • These findings contribute to understanding reserpine's neuropharmacological effects.