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Temperature dependency of P2 receptor-mediated responses
Airat U Ziganshin1, Alexey V Rychkov, Lilia E Ziganshina
1Department of Pharmacology, Kazan State Medical University, Kazan, Russia. airatz@auz.kcn.ru
European Journal of Pharmacology
|November 27, 2002
Summary
Lowering temperature enhances P2 receptor responses in guinea pig urinary bladder and vas deferens. This study investigated temperature effects on P2X and P2Y receptor activity in smooth muscle tissues.
Area of Science:
- Pharmacology
- Physiology
- Neuroscience
Background:
- P2 receptors are involved in smooth muscle function.
- Temperature can influence receptor-mediated physiological responses.
Purpose of the Study:
- To investigate the effect of temperature on P2 receptor-mediated responses in guinea pig urinary bladder, vas deferens, and taenia caeci.
- To characterize the temperature-dependence of P2X and P2Y receptor activity.
Main Methods:
- Isolated guinea pig urinary bladder, vas deferens, and taenia caeci were used.
- Responses to P2 receptor agonists and electrical field stimulation were measured at 30, 37, and 42 degrees C.
- The effects of P2 receptor antagonist PPADS were assessed at different temperatures.
Main Results:
- Contractile responses to P2X receptor agonist alpha,beta-methylene ATP and electrical field stimulation were more prominent at 30 degrees C.
- Temperature-dependent relaxation of taenia caeci by electrical field stimulation increased with decreasing temperature.
- Relaxation by exogenous ATP was not affected by temperature, and PPADS antagonized P2X receptor responses without temperature dependence.
Conclusions:
- The effectiveness of P2 receptor-mediated responses in guinea pig urinary bladder, vas deferens, and taenia caeci increases with decreasing temperature.
- Temperature significantly modulates P2X receptor activity in these tissues.
- P2Y receptor-mediated relaxation by ATP is less sensitive to temperature changes.