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Separation methods applicable to urinary creatine and creatinine.

Truis Smith-Palmer1

  • 1Department of Chemistry, St. Francis Xavier University, Antigonish, NS, Canada B2G 2W5. tsmithpa@stfx.ca

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|November 27, 2002
PubMed
Summary

Measuring urinary creatine and creatinine is crucial for assessing muscle health and kidney function. New methods enhance the analysis of these compounds in urine, serum, and tissue.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Chemistry

Background:

  • Urinary creatinine is a long-standing biomarker for glomerular filtration rate.
  • Creatine supplementation is prevalent among athletes, necessitating urinary creatine measurement.
  • Elevated urinary creatine can indicate muscle degenerative diseases.

Purpose of the Study:

  • To review methods for analyzing urinary creatine and creatinine.
  • To highlight the importance of these analytes in clinical and sports contexts.

Main Methods:

  • High-Performance Liquid Chromatography (HPLC) with various detection methods (absorption, fluorescence, mass spectrometry).
  • Automated analytical techniques.
  • Capillary Electrophoresis (CE) methods, including capillary zone electrophoresis and micellar electrokinetic capillary chromatography.

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Main Results:

  • HPLC and CE are effective for analyzing both creatine and creatinine in urine.
  • Methods are adaptable for serum and tissue analysis.
  • Automation of some analytical procedures is available.

Conclusions:

  • Accurate measurement of urinary creatine and creatinine is vital for monitoring kidney function and muscle health.
  • Diverse analytical techniques, including HPLC and CE, are suitable for these measurements.
  • Further application of these methods to serum and tissue analysis is feasible.