Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydroxyl radical in living systems and its separation methods.

Fu-Chou Cheng1, Jen-Fon Jen, Tung-Hu Tsai

  • 1Department of Medical Research, Taichung Veterans General Hospital, Taichung 407, Taiwan.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|November 27, 2002
PubMed
Summary

Hydroxyl radicals are crucial in health and disease, linked to oxidative stress. This study reviews analytical methods for detecting these reactive oxygen species and their role in various pathologies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The anti-COVID-19 drug Paxlovid crosses biological barriers of the placenta and brain in rats.

Npj viruses·2025
Same author

The anti-COVID-19 drug nirmatrelvir crosses the blood‒brain barrier and exhibits herb-drug pharmacokinetic interactions with <i>Scutellaria baicalensis</i> formulations.

Heliyon·2024
Same author

Cordycepin Augments the Efficacy of Anti-PD1 against Colon Cancer.

Biomedicines·2024
Same author

Multi-microdialysis analytical system to monitor acetaminophen and its pharmacokinetic interaction with A. bidentata in rat blood, forelimb extensor muscle, brain striatum, and the knee joint cavity.

Journal of pharmaceutical and biomedical analysis·2024
Same author

FK228 suppress the growth of human malignant pleural mesothelioma tumor independent to epithelioid or non-epithelioid histology.

Molecular medicine (Cambridge, Mass.)·2024
Same author

Pharmacokinetic analysis of antiviral drug ritonavir across the blood-brain barrier and its interaction with Scutellaria baicalensis using multisite microdialysis in rats.

Journal of pharmaceutical and biomedical analysis·2024

Area of Science:

  • Biochemistry
  • Cellular Biology
  • Pathology

Background:

  • Hydroxyl radicals are generated in physiological and pathological states, linked to oxidative stress.
  • These radicals are vital for cellular homeostasis but implicated in disease when in excess.
  • Excess hydroxyl radicals or deficient antioxidant defenses cause cellular damage.

Purpose of the Study:

  • To review analytical methods for hydroxyl radical detection.
  • To discuss the role of hydroxyl radicals in disease pathogenesis.
  • To explore novel separation techniques for hydroxyl radical analysis.

Main Methods:

  • Liquid chromatography with electrochemical detection and mass spectrometry
  • Gas chromatography with mass spectrometry

Related Experiment Videos

  • Capillary electrophoresis, electron spin resonance, and chemiluminescence

    • Main Results:

    • Detailed information on various hydroxyl radical trapping and detection techniques is provided.
    • The paper discusses the intricate relationship between diseases and hydroxyl radical generation.
    • Novel separation methods for hydroxyl radical analysis are presented.

    Conclusions:

    • Accurate analytical methods are essential for understanding hydroxyl radical's role in biological systems.
    • Hydroxyl radical detection and quantification are critical for disease research.
    • Further research into novel separation techniques will advance the field.