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Competitive PCR for quantitation of a Cytophaga-Flexibacter-Bacteroides phylum bacterium associated with the Tuber

Elena Barbieri1, Giulia Riccioni, Anna Pisano

  • 1Giorgio Fornaini Institute of Biochemistry, University of Urbino, Pesaro-Urbino, Italy.

Applied and Environmental Microbiology
|November 27, 2002
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...