Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Directed evolution of a genetic circuit.

Yohei Yokobayashi1, Ron Weiss, Frances H Arnold

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, 210-41, 1200 East California Boulevard, Pasadena, CA 91125, USA.

Proceedings of the National Academy of Sciences of the United States of America
|November 27, 2002
PubMed
Summary

Biological circuit engineers can now fine-tune synthetic genetic networks using a combined rational and evolutionary design strategy. This approach rapidly evolves nonfunctional circuits into functional ones, creating a library of genetic devices for complex circuitry.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering drug-responsive replication machinery for precise control of self-amplifying RNA.

Nature biomedical engineering·2026
Same author

Current Progress and Future Outlook for Synthetic Gene Circuits in Cardiovascular Therapy.

Biomolecules·2026
Same author

Engineering Enantiocomplementary Protoglobins for Stereoconvergent Construction of <i>N</i>-Alkylated α-Aminoketones.

Journal of the American Chemical Society·2026
Same author

ETV2 Mediated Differentiation of Human Pluripotent Stem Cells Results in Functional Endothelial Cells for Engineering Advanced Vascularized Microphysiological Models.

Advanced healthcare materials·2026
Same author

A Chemoselective and Stereodivergent Platform of Heme-Nitrene Transferases to Access Chiral Aryl-β-Amino Esters and An Investigation of the Sequence-Activity Landscape.

Angewandte Chemie (International ed. in English)·2026
Same author

Sequence-based generative AI design of versatile tryptophan synthases.

Nature communications·2026

Area of Science:

  • Synthetic biology
  • Genetic engineering
  • Biological circuit design

Background:

  • Predicting the behavior of synthetic biological networks remains a significant challenge for engineers.
  • Designing complex genetic circuitry requires overcoming limitations in current prediction models.

Purpose of the Study:

  • To develop a combined rational and evolutionary design strategy for constructing functional genetic regulatory circuits.
  • To enable in vivo experimental fine-tuning of biochemical parameters in biological networks.

Main Methods:

  • Applying directed evolution to genes within a simple genetic circuit.
  • Utilizing a combined rational and evolutionary design approach for genetic regulatory circuits.

Main Results:

Related Experiment Videos

  • A nonfunctional genetic circuit with mismatched components rapidly evolved into a functional one.
  • A library of genetic devices with diverse behaviors was generated through directed evolution.

Conclusions:

  • The proposed strategy allows for the fine-tuning of synthetic biological networks.
  • The generated library of genetic devices facilitates the construction of more complex biological circuits.