Patterned polymer surfaces for cell culture applications
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Updated: Sep 28, 2026
A Versatile Method of Patterning Proteins and Cells
Published on: February 26, 2017
A Welle1, E Gottwald, K F Weibezahn
1Institut für Medizintechnik und Biophysik, Forschungszentrum Karlsruhe GmbH, Deutschland. Alexander.Welle@imb.fzk.de
Abstract:
We studied the physico/chemical effects of deep UV irradiation of polystyrene, PMMA and polycarbonate with respect to cell adhesion and protein immobilization. Photochemical modifications of the polymer surfaces yielded unstable peroxides and carboxylic acid groups. Patterned enzyme and antibody adsorbates were realized by coupling via carbodiimid activation of the COOH-moities. Hepatoma cells (HepG2) and fibroblasts (L929) adhered in the presence of serum proteins in the culture medium on the irradiated regions of the substrate without any further treatment.
