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[Effect of EMP-1 gene on human esophageal cancer cell line]
Hai-tao Wang1, Zhi-hua Liu, Xiu-qin Wang
1National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100021, P. R. China.
Background & Objective:
EMP-1 was selected from a series of differential expressed genes obtained from cDNA microarray in the authors' lab. Epithelial membrane pnteiu-1 gene (EMP-1) was expressed 6 fold lower in esophageal cancer than in normal tissue. The authors further designed the experiment to study the effect of human EMP-1 gene on human esophageal cancer cell line in order to explain the function of this gene on the carcinogensis and progression esophageal cancer.
Methods:
EMP-1 gene was cloned into eukaryotic vector and transfected into the human esophageal cancer cell line. The transfection effect was qualified by Western blot and RT-PCR method. The cell growth curve was observed and the cell cycle was checked by FACS method.
Results:
EMP-1 was transfected into EC9706 cell line and its expression was up-regulated. The cell growth is accelerated and expression of EMP-1 is linked to induction of S phase arrest.
Conclusion:
EMP-1 gene has some relationship with carcinogenesis of esophagus.