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Published on: March 18, 2015
[Comparison of antitumor efficacy between arsacetyl and arsenic trioxide in vitro]
Feng-ling Huang1, Yuan-liang Wang, Chun-xiao Chen
1Department of Biomedcine Engineering, College of Automatization, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, P. R. China. Hfengling@263.net
Background & Objective:
Recently, it was reported that some organic arsenics was stronger than inorganic arsenics in inducing carcinoma cell apoptosis. This study was designed to compare arsacetyl (ASAC), a kind of organic arsenics, with As2O3 in the effect of inhibiting proliferation and inducing apoptosis in carcinoma cells.
Methods:
MTT (Methythiazolyl tetrazolium) assay and analysis of apoptosis were used to evaluate the effects of arsacetyl and As2O3 on the proliferation of SMMC-7721 and their mechanisms. Their toxicity to vessel endothelium cells (VECs) was also determined by using MTT method.
Results:
Both As2O3 and arsacetyl significantly inhibited the proliferation of SMMC-7721, the inhibitory effect was dose- and time-dependent and arsacetyl was stronger than As2O3 [e.g. inhibitory ratio: (14.2 +/- 1.5)%, treated with 0.1 mumol/L arsacetyl for 48 h; (27.1 +/- 3.0)%, treated with 0.1 mumol/L arsacetyl for 48 h]. 1 mumol/L As2O3 and 0.1 mumol/L arsacetyl almost had no toxicity to VEC, while they induced SMMC-7721 apoptosis indicated morphologically by "small apoptopic body" formation; DNA ladders appeared on agarose gel electrophoresis. The flow cytometry assay indicated that most of the cells were arrested in S + G2/M phase and the apoptotic peak appeared.
Conclusion:
The ability of arsacetyl inducing apoptosis was stronger than As2O3, and SMMC-7721 in the G0/G1 phase may be the target of drug action.
