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[Expression of Fas in six human urogenital malignant cell lines]
Hong-jun Li1, Li-zhang Yu, Ying-lu Guo
1Daxing Male Hospital of Wu Jieping's Medical Foundation, Beijing 102600, P. R. China. hongjun63@btamail.net.cn
Background & Objective:
Expression of Fas in tumors is an important basis for the treatment of the patients with tumor and determination of the prognosis. This study was designed to investigate the expression of Fas in urogenital tumor cell lines.
Methods:
Using direct immunofluorescence flow cytometry, Western blot, we detected and Northern blot, the expression of Fas in six urinary malignant cell lines [bladder carcinoma(T24, EJ, BIU-87), renal carcinoma (GRC-1, RCC-949), prostatic carcinoma (PC-3M)] and one primary in vitro cultured renal fibroblast.
Results:
Expression of Fas in 6 urinary tumor cell lines was commonly presented but with limited positive cell percentage (range 16.51%-49.13%) and relatively lower fluorescence intensity, compared with expression of Fas in normal control of primary in vitro cultured renal fibroblast (the positive rate of Fas reached 77.98%) measured by flow cytometry with direct immunofluorescence; In detection of expression of Fas in 6 urogenital cell lines, a 48 kDa clear band was found with the strongest intensity in T24 cell line and the weakest in BIU-87. Fas mRNA from all tumor cell lines but BIU-87 was detected.
Conclusions:
Fas was extensively expressed in urogenital malignant cells and normal cell. The lower expression of Fas in urinary malignant cell lines than that in normal cells might be the reason for occurrence and progression of urinary malignant tumors.