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Updated: Aug 2, 2026
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
[Effect of soluble vascular endothelial growth factor (VEGF) receptor gene (sflt-1) and antisense VEGF nucleotide on
Jun Mi1, Jian-hua Wang, Shi-shu Chen
1Department of Biochemistry & Molecule Biology, Shanghai Second Medical University, Shanghai 200025, P. R. China.
Background & Objectives:
Nutrients and growth factor provided by new blood vessel in tumor tissue is critical for fast growth of tumor, therefore, how to inhibit neovascularization and speedup neocrosis of tumor tissue is a promising way to treat tumor. This article emphasizes on both the blockage effect of soluble VEGF receptor gene (sflt-1) and antisense VEGF nucleotide on VEGF in tumor tissue, in order to inhibit its neovascularization.
Methods:
Effect of antisense VEGF on VEGF secretion in MM45T. Li cells was observed after infection with Ad-antisense VEGF; Effect of recombinant soluble VEGF receptor protein (3' delta Flt-1) on proliferation of (human umbilical vascular endothelial cell) HUVEC induced by VEGF was investigated; Effect of both antisense VEGF and sflt-1 on neovascularization were tested after Ad-anti VEGF or Ad-sflt-1 injected in chorion of chicken embryo.
Results:
VEGF concentration from infected MM45T. Li by recombinant Ad-antisense VEGF is only 15% of that from control group (P < 0.01); the proliferation of HUVEC induced by VEGF in condition medium containing 3' delta Flt-1 is significantly reduced, and negatively related with dose within certain range; Both sflt-1 and antisense VEGF nucleotide obviously inhibited formation of new blood vessel, even caused dead embryo.
Conclusion:
Both sflt-1 and antisense VEGF could effectively inhibited neovascularization separately, combination therapy can enhance the inhibitory effect, but the mechanism is different.
Insights
Both soluble VEGF receptor (sflt-1) and antisense VEGF nucleotide effectively inhibit tumor neovascularization. Combination therapy enhances this effect, offering a promising strategy for cancer treatment by blocking tumor blood vessel formation.
Area of Science:
- Molecular Biology
- Oncology
- Gene Therapy
Context:
- Tumor growth relies on nutrients from new blood vessels (neovascularization).
- Inhibiting tumor neovascularization is a key cancer treatment strategy.
- Vascular Endothelial Growth Factor (VEGF) drives tumor angiogenesis.
Purpose:
- To investigate the anti-angiogenic effects of soluble VEGF receptor gene (sflt-1) and antisense VEGF nucleotide.
- To evaluate the combined efficacy of sflt-1 and antisense VEGF in inhibiting tumor neovascularization.
Summary:
- Ad-antisense VEGF significantly reduced VEGF secretion in MM45T. Li cells (15% of control).
- Recombinant soluble VEGF receptor protein (3' delta Flt-1) inhibited VEGF-induced human umbilical vascular endothelial cell proliferation.
- Both sflt-1 and antisense VEGF markedly inhibited neovascularization in chicken embryos, with combination therapy showing enhanced effects.
Impact:
- Demonstrates the potential of sflt-1 and antisense VEGF as anti-angiogenic agents.
- Highlights the synergistic effect of combining these two therapeutic approaches.
- Provides a basis for developing novel cancer therapies targeting tumor angiogenesis.
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