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Elevated tissue plasminogen activator as a potential marker of endothelial dysfunction in pre-eclampsia: correlation
Luís Belo1, Alice Santos-Silva, Ann Rumley
1Department of Biochemistry, Faculty of Pharmacy, University of Porto, Portugal.
Objective:
To clarify the role played by endothelial cell production of fibrinolytic factors in normal pregnancy and pre-eclampsia.
Design:
A longitudinal study performed during normal pregnancy and a cross sectional study performed in healthy and pre-eclamptic pregnant women in the third trimester of pregnancy.
Setting:
Department of Obstetrics and Gynaecology, University Hospital of S. João, Porto, Portugal.
Population:
Fourteen normal pregnant women followed through the three trimesters of gestation. Two groups of women (normal, n = 56; pre-eclamptic, n = 37) evaluated at the third trimester of gestation.
Methods:
Measurement of platelet number, plasma levels of fibrinogen, tissue plasminogen activator (t-PA) antigen, plasminogen activator inhibitor-1 (PAI-1) activity, and fibrin fragment D-dimer.
Main Outcome Measures:
Pre-eclampsia, proteinuria.
Results:
All the substances, except platelet count, increased significantly throughout normal pregnancy. Comparison of the values in the third trimesters of normal and pre-eclamptic pregnancies showed similar values for the fibrinogen and platelet counts, and higher values of t-PA (almost twice normal median value; P < 0.0001), PAI-1 and D-dimer in the pre-eclamptic women. t-PA correlated positively and significantly with the degree of proteinuria in pre-eclamptic women (r = 0.575, P = 0.0002).
Conclusion:
These findings suggest that elevated t-PA antigen may reflect endothelial disturbance in preeclampsia, and may be a potential biomarker of risk.