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Evaluation of platelet parameters on the ADVIA 120 as the quality indicator for stored platelets
1Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea. limyoung@madang.ajou.ac.kr
Insights
Platelet component distribution width (PCDW) effectively monitors platelet storage quality. This simple indicator correlates with platelet activation and functional reserve, aiding transfusion viability assessments.
Area of Science:
- Hematology
- Transfusion Medicine
- Biotechnology
Background:
- Platelet concentrates require robust quality control during storage.
- Assessing platelet viability and storage lesions is crucial for transfusion efficacy.
- Current methods may not be sufficiently routine or cost-effective.
Purpose of the Study:
- To evaluate platelet parameters from the ADVIA 120 hematologic analyzer.
- To determine their utility as routine quality control indicators for stored platelets.
- To correlate these parameters with platelet function and storage.
Main Methods:
- Stored platelet concentrates were analyzed over 5 days.
- Measurements included platelet parameters (PCDW, MPC), pH, and CD62P expression.
- CD62P expression was assessed on unstimulated and stimulated platelets.
Main Results:
- Platelet component distribution width (PCDW) showed a progressive decrease during storage.
- PCDW correlated with CD62P expression, indicating platelet activation and reduced functional reserve.
- Mean platelet component (MPC) decreased initially but did not show a progressive trend or correlate with CD62P.
Conclusions:
- PCDW is a valuable, cost-effective indicator for assessing platelet viability and storage lesions.
- PCDW offers a simple and convenient method for routine quality control of platelet preparations.
- This parameter aids in ensuring the quality of platelets for transfusion.
Abstract:
In order to investigate the possible use of platelet parameters on the ADVIA 120 hematologic analyzer as the routine quality control indicator for preparation and storage of platelets, platelet parameters, pH and CD62P expression were determined in stored platelet concentrates. Platelet component distribution width (PCDW) was decreased progressively on days 1 and 3 of storage for 5 days when compared with 0 day. PCDW correlated with CD62P expression on unstimulated platelets and the difference in CD62P expression following agonistic stimulation (a measure of functional reserve). Mean platelet component (MPC) was decreased on day 1 of storage. It did not however, show a progressive decrease over storage time and did not correlate with CD62P, although MPC has been known to be a useful screening test for platelet activation. Therefore, PCDW is considered to be a simple, convenient and cost-effective quality indicator for determining the viability and storage lesion of platelets for transfusion.
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