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Corneal epithelial changes during midterm storage.

R A Thoft, J Friend

    Investigative Ophthalmology
    |February 1, 1976
    PubMed
    Summary
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    Donor corneal epithelium viability during storage is crucial for keratoplasty. Studies show epithelial cells can recover vital substances like glycogen and ATP after midterm storage, even with initial depletion.

    Area of Science:

    • Ophthalmology
    • Corneal Transplantation
    • Cellular Metabolism

    Background:

    • Keratoplasty relies on donor corneal tissue.
    • Donor epithelium viability is critical for successful transplantation outcomes.
    • Midterm storage methods impact epithelial cell health.

    Purpose of the Study:

    • To assess corneal epithelial cell viability during midterm storage.
    • To evaluate the impact of different storage conditions on epithelial metabolic indicators.
    • To determine the recovery potential of epithelial cells after storage.

    Main Methods:

    • Measured glycogen, ATP, and ADP levels in corneal epithelium.
    • Compared conventional moist-chamber storage with McCarey-Kaufman medium immersion.
    • Assessed metabolic indicator recovery after warming to 37°C.

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  • Evaluated storage durations of 1, 2, and 12 days at 4°C.

    • Main Results:

    • Epithelial cells showed depletion of glycogen and ATP during storage.
    • Corneal tissue demonstrated recovery of glycogen and ATP stores after warming.
    • Storage conditions influenced the degree of metabolic depletion and recovery.

    Conclusions:

    • Corneal epithelium retains the capacity for metabolic recovery post-storage.
    • This finding is significant for optimizing donor eye preservation techniques.
    • Donor epithelium viability can be maintained through appropriate storage and rewarming protocols.