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Related Experiment Videos

Stem cell factor induces eosinophil activation and degranulation: mediator release and gene array analysis.

Sandra H P Oliveira1, Dennis D Taub, James Nagel

  • 1Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, USA.

Blood
|November 28, 2002
PubMed
Summary

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Stem cell factor (SCF) activates mouse eosinophils, causing them to release inflammatory mediators and up-regulate over 150 genes, including chemokines. This research highlights SCF

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Eosinophils are key effector cells in allergic reactions, releasing mediators.
  • Stem cell factor (SCF) is crucial for hematopoiesis and mast cell function.
  • SCF is implicated in allergic airway inflammation pathogenesis.

Purpose of the Study:

  • To investigate SCF's role in activating murine eosinophils.
  • To assess SCF's effect on mediator release and chemokine up-regulation.
  • To identify genes modulated by SCF stimulation in eosinophils.

Main Methods:

  • Murine eosinophils were stimulated with SCF.
  • Mediator release (e.g., eosinophil peroxidase, leukotriene C4) was measured.
  • Gene array analysis and quantitative real-time PCR were performed.

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Main Results:

  • Eosinophils express the SCF receptor, c-kit.
  • SCF induced dose-dependent degranulation and release of inflammatory mediators.
  • SCF upregulated CC chemokines (RANTES, MDC, MIP-1beta, C10) and over 150 inflammation-related genes.
  • Gene array findings were validated by PCR.

Conclusions:

  • SCF is a potent activator and degranulator of eosinophils.
  • SCF plays a significant role in eosinophil-mediated inflammatory and immune responses.
  • SCF may be a therapeutic target in allergic inflammatory diseases.