Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell adhesiveness and affinity for limb pattern formation.

Hiroshi Yajima1, Kenji Hara, Hiroyuki Ide

  • 1Biological Institute, Graduate School of Life Science, Tohoku University, Aobayama Aoba-ku, Sendai, Japan.

The International Journal of Developmental Biology
|November 29, 2002
PubMed
Summary

N-cadherin mediates stage-dependent cell sorting in chick limb buds, influencing proximal-distal patterning. This cell adhesion molecule is crucial for establishing positional identity during limb development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The clinical significance of SIGLEC1 mRNA levels derived from peripheral leukocytes in autoimmune thyroid diseases and type 1 diabetes.

Endocrine journal·2026
Same author

Radiation Recall Mucositis After Administration of Tegafur-Gimeracil-Oteracil Potassium (TS-1).

The Journal of dermatology·2026
Same author

Visualization of peripheral nerves in developing and regenerating limbs using a novel peripherin reporter line of Xenopus laevis.

Biology open·2026
Same author

Acute onset of neovascular age-related macular degeneration after initiation of tirzepatide.

Diabetology international·2026
Same author

Pretreatment Cytokine Signatures as Candidate Biomarkers for JAK Inhibitor Response in Anti-MDA5 Dermatomyositis-Related Interstitial Lung Disease: A Pilot Study.

ACR open rheumatology·2026
Same author

A Pediatric Case of KRT2 Nonsyndromic Epidermal Differentiation Disorder With a Concurrent GJB4 Variant.

The Journal of dermatology·2026

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Mesenchymal cells in chick limb buds exhibit stage-dependent cell sorting in vitro.
  • N-cadherin, a cell adhesion molecule, was previously implicated in limb bud cell sorting and proximal-distal patterning.

Purpose of the Study:

  • To provide direct evidence for N-cadherin's role in regulating stage-dependent cell sorting in vitro.
  • To explore the relationship between N-cadherin accumulation and positional identity in chick limb development.

Main Methods:

  • In vitro cell sorting assays using chick limb bud mesenchymal cells.
  • Analysis of N-cadherin expression patterns during limb development.
  • Investigation of the role of Hox genes and cell surface properties in positional identity.

Related Experiment Videos

Main Results:

  • N-cadherin directly regulates stage-dependent cell sorting in vitro.
  • N-cadherin accumulates in the distal chick limb bud as development progresses.
  • N-cadherin is linked to positional identity, affecting the formation of proximal-distal skeletal elements.

Conclusions:

  • N-cadherin is a key molecule in regulating cell sorting during chick limb development.
  • The accumulation of N-cadherin in the distal limb bud contributes to establishing positional identity.
  • Hox genes and cell surface properties, alongside N-cadherin, mediate positional identity in limb development.