Conserved features of endocytosis in Dictyostelium

Endocytosis in protozoa is often regarded as largely different from the pathways operating in mammalian cells. Experiments in the amoeba Dictyostelium, one of the genetically tractable single-celled organisms, have allowed us to manipulate the flow through endocytic compartments and to study the dynamic distribution of molecules by means of green fluorescent protein fusions. This review attempts to compile the molecular data available from Dictyostelium and assign them to specific steps of internalization by phagocytosis or macropinocytosis and to subsequent stages of the endocytic pathway. Parallels to phagocytes of the mammalian immune system are emphasized. The major distinctive feature between mammalian phagocytes and free-living cells is the need for osmoregulation. Therefore Dictyostelium cells possess a contractile vacuole that has occasionally obscured analysis of endocytosis but is now found to be entirely separate from endocytic organelles. In conclusion, the potential of Dictyostelium amoebas to provide a model system of mammalian phagocytes is ever increasing.