Automated fatty acid analysis

An automated system for the analysis of fatty acids is described. Samples dissolved in CS2 are automatically injected and separated by temperature programmed gas chromatography. Peak areas and retention times were measured by an electronic integrator, and recorded on punched paper tape. Peaks are identified and amounts calculated by an off-line computer program based on one or more internal standards. The system is designed for use with samples of at least 10 mug. Some of the problems adn limitations are discussed.