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Aspergillus antigens associated with allergic bronchopulmonary aspergillosis

Bhanu P Singh1, Banani Banerjee, Viswanath P Kurup

  • 1Centre For Biochemical Technology, Delhi, India. singhbp1951@yahoo.com

Frontiers in Bioscience : a Journal and Virtual Library
|November 29, 2002
PubMed

Insights

Aspergillus fumigatus (Af) is a common mold allergen. Researchers are improving diagnostic methods for allergic aspergillosis using advanced Af antigen purification techniques.

Area of Science:

  • Allergen immunotherapy
  • Medical mycology
  • Immunodiagnostics

Background:

  • * Aspergillus fumigatus (Af) is a prevalent mold allergen found indoors and outdoors.
  • * Antigens from Af are used for diagnosing allergic aspergillosis.
  • * Crude Af extracts present challenges in diagnosis due to impurities and cross-reactivity.

Purpose of the Study:

  • * To review advancements in Af allergen purification.
  • * To highlight the development of specific and reliable immunoassays.
  • * To address the lack of international standards in Af allergen testing.

Main Methods:

  • * Isolation of secreted and bound antigens from Af.
  • * Use of crude extracts for in vitro IgE antibody detection and skin testing.
  • * Application of monoclonal antibody-based affinity purification and molecular biology techniques.
  • * Conventional purification and fractionation methods.

Main Results:

  • * Crude extracts show inconsistent reactivity and cross-reactivity with other allergens.
  • * Advanced methods like affinity purification and molecular biology have improved allergen purification.
  • * Development of more specific and reliable immunoassays is ongoing.

Conclusions:

  • * Purification of Af allergens is crucial for accurate immunodiagnosis.
  • * Modern techniques offer significant progress in developing reliable diagnostic tools.
  • * Establishment of international standards is necessary for consistent laboratory results.

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