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Molecular characterization of foot-and-mouth disease virus O/SKR/2000
Chang Hee Kweon1, Young Joon Ko, Won Il Kim
1Virology Research Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, South Korea. kweonch@mail.nvrqs.go.kr
Abstract:
Molecular cloning and sequencing of the genome of foot-and-mouth disease virus (FMDV) O/SKR/2000, one of PanAsia strain, were performed from FMDV infected cattle. From the poly (C) tract of the 5' nontranslated region (NTR) to the 3' NTR including 14 base pairs (bp) of poly (A) tail, 7813 bp sequences comprising approximately 95% of the whole genome were obtained by reverse transcription polymerase reaction (RT-PCR). The deduced amino acid sequences of the structural and nonstructural proteins (NSP) of the O/SKR/2000 virus were analyzed for the sequence similarity among type O strains. Comparison between FMDV O/SKR/2000 and other strains indicates that overall the number of amino acids appears to be conserved without any deletion in either NSP or capsid proteins, thus, suggesting that O/SKR/2000 evolved with minor difference from preexisting strains.
Insights
Researchers sequenced the genome of the foot-and-mouth disease virus (FMDV) O/SKR/2000 PanAsia strain. The analysis revealed conserved amino acid sequences, suggesting minor evolution from existing strains.
Area of Science:
- Virology
- Genomics
- Molecular Biology
Background:
- Foot-and-mouth disease virus (FMDV) poses a significant threat to livestock globally.
- Understanding viral genome evolution is crucial for effective disease control.
Purpose of the Study:
- To perform molecular cloning and sequencing of the FMDV O/SKR/2000 PanAsia strain genome.
- To analyze the sequence similarity of structural and nonstructural proteins (NSP) with other FMDV type O strains.
Main Methods:
- Reverse transcription polymerase reaction (RT-PCR) was used to obtain approximately 95% of the viral genome sequence.
- Sequencing covered the 5' nontranslated region (NTR) to the 3' NTR, including the poly (A) tail.
- Deduced amino acid sequences of proteins were analyzed for similarity.
Main Results:
- A total of 7813 bp of the FMDV O/SKR/2000 genome was sequenced.
- Analysis showed conserved amino acid sequences in both NSP and capsid proteins.
- No significant deletions were observed in the O/SKR/2000 strain compared to other type O strains.
Conclusions:
- The FMDV O/SKR/2000 strain exhibits high sequence conservation with existing type O strains.
- This suggests that the O/SKR/2000 strain evolved with minimal genetic differences.
- The findings contribute to understanding FMDV evolution and strain diversity.