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Expression of gene encoding P65 oncofetal protein in acute and chronic leukemias
E Balcerczak1, J Bartkowiak, J Z Błoński
1Department of Pharmaceutical Biochemistry, Molecular Biology Laboratory; Medical University, Lodz, 90-151 Poland. mirowski@ich.pharm.am.lodz.pl
Abstract:
In this study we have established conditions for p65 gene expression analysis by reverse transcriptase polymerase chain reaction (RT-PCR). On the basis of this technique we analyzed p65 gene expression in various types of leukemia: acute myeloblastic leukemia (AML) (n=26); acute lymphoblastic leukemia (ALL) (n=26) and chronic lymphocytic leukemia (CLL) (n=40). The highest frequency of p65 gene expression was found in the patients with CLL (66%). No relationship between the expression of p65 gene and clinical stage of leukemia was observed. The lower percentage of positivity (presence of gene transcript) was seen in patients with ALL (42%) and AML (46%).