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Effect of murine leukaemia virus age on its 8S RNA components
The Journal of General Virology
|January 1, 1976
Abstract:
The effect of virion age on the two A and B 8 S RNA conformational isomers was investigated by electrophoresis on polyacrylamide gels. The relative proportions of A and B 8 S RNA components of the immature virus (rapid harvest, 30 min) are intermediate between those previously found for the mature virus and for the host cell. These results suggest that a change of 8S RNA conformation occurs during virus maturation.
Insights
Virion age impacts 8S RNA conformation. Immature virus RNA isomers are intermediate between mature virus and host cell states, suggesting conformational changes during maturation.
Area of Science:
- Molecular Biology
- Virology
- Biochemistry
Background:
- The study investigates the conformational isomers of 8S RNA within virus particles.
- Understanding RNA conformation is crucial for comprehending viral replication and assembly.
Purpose of the Study:
- To determine the effect of virion age on the conformational isomers of 8S RNA.
- To elucidate the role of 8S RNA conformational changes during the virus maturation process.
Main Methods:
- Polyacrylamide gel electrophoresis was employed to analyze RNA components.
- The relative proportions of A and B 8S RNA isomers were quantified in immature and mature viruses, as well as host cells.
Main Results:
- The proportions of A and B 8S RNA in immature virions (30 min harvest) were found to be intermediate.
- These intermediate proportions fall between those observed in mature virions and the host cell.
Conclusions:
- A conformational change in 8S RNA occurs during the maturation of the virus.
- Virion age is a significant factor influencing 8S RNA conformation.