Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new method for fish leucocyte counting and partial differentiation by flow cytometry.

Takamasa Inoue1, Tadaaki Moritomo, Yumi Tamura

  • 1Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252-8510 Japan.

Fish & Shellfish Immunology
|December 3, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD80 (B7-1) as a potential therapeutic target in Epstein-Barr virus‑associated B cell diseases.

Scientific reports·2026
Same author

Development of an antigen-specific CD4-1<sup>+</sup> T cell response assay based on cytokine gene expression in ginbuna crucian carp (Carassius auratus langsdorfii).

Fish & shellfish immunology·2026
Same author

T cell-mediated immune surveillance conferred by latent Epstein-Barr virus genes suppresses a broad spectrum of tumor formation through NKG2D-NKG2DL interactions.

Frontiers in immunology·2025
Same author

Co-expression of B7-H3 and LAG3 represents cytotoxicity of CD4<sup>+</sup> T cells in humans.

Frontiers in immunology·2025
Same author

Establishment of a novel clonal GFP-expressing transgenic ginbuna crucian carp.

Developmental and comparative immunology·2024
Same author

Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment.

Fish & shellfish immunology·2024

This study presents a rapid flow cytometry method for analyzing carp blood cells. The technique accurately identifies five distinct fish blood cell populations using fluorescent staining and scatter properties.

Area of Science:

  • Aquatic biology
  • Hematology
  • Flow cytometry

Background:

  • Accurate enumeration of fish blood cells is crucial for health assessment.
  • Traditional microscopy methods can be time-consuming and labor-intensive.

Purpose of the Study:

  • To develop a simple, rapid, and accurate flow cytometry method for fish blood cell analysis.
  • To identify and differentiate various fish blood cell populations.

Main Methods:

  • Carp blood was diluted and stained with a fluorescent lipophilic dye (DiOC6(3)).
  • Cells were analyzed using a flow cytometer (FACS), measuring green fluorescence (FL-1), forward scatter (FSC), and side scatter (SSC).
  • Dot-plots of FL-1 vs. SSC and FSC vs. SSC were used for cell population identification.

Related Experiment Videos

Main Results:

  • Five distinct fish blood cell populations were identified: erythrocytes, thrombocytes/lymphocytes, monocytes, neutrophils, and basophils.
  • The flow cytometry counts showed good agreement with traditional microscopic counts.
  • The method demonstrated high efficiency in differentiating cell types based on fluorescence and scatter characteristics.

Conclusions:

  • Flow cytometry offers a fast and reliable alternative for fish hematological analysis.
  • This method facilitates accurate identification and quantification of carp blood cell types.
  • The developed technique has potential applications in fish health monitoring and research.