Related Experiment Video
Updated: Aug 5, 2026
08:40
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
P504S immunohistochemical detection in 405 prostatic specimens including 376 18-gauge needle biopsies
R Beach1, A M Gown, M N De Peralta-Venturina
1Department of Pathology and Laboratory Medicine, Emory University Hospital, 1364 Clifton Road NE, Atlanta, GA 30322, USA.
The American Journal of Surgical Pathology
|December 3, 2002
Summary
Immunohistochemical detection of P504S protein is a valuable tool for diagnosing prostate cancer, even after hormone or radiation therapy. Specific staining patterns are key, but P504S should be used with microscopic correlation.
Area of Science:
- Uropathology
- Molecular Pathology
- Cancer Biomarkers
Background:
- P504S is a prostate cancer-specific gene.
- Its protein product is involved in fatty acid beta-oxidation.
- Immunohistochemical detection of P504S is a sensitive and specific marker for prostatic carcinoma.
Purpose of the Study:
- To analyze P504S protein expression in various prostate and bladder specimens.
- To evaluate P504S staining in specific morphologic patterns of prostatic adenocarcinoma, post-treatment cases, and invasive urothelial carcinoma.
Main Methods:
- A total of 366 prostate needle core biopsies and 28 prostatectomy specimens were stained with P504S monoclonal antibody.
- Standard immunohistochemical techniques were employed.
- Extent and location of immunoreactivity were recorded in carcinoma and benign tissues.
Main Results:
- 82% of prostatic adenocarcinoma biopsies stained positive for P504S.
- Staining patterns were consistent across different morphologic types and post-treatment cases.
- 21% of benign prostatic tissue showed focal, faint staining; seminal vesicles were negative.
- 83% of invasive urothelial carcinoma specimens showed significant P504S staining.
Conclusions:
- P504S immunohistochemistry is useful for diagnosing prostate cancer, including treated cases.
- Circumferential luminal to subluminal and diffuse cytoplasmic staining is highly specific for prostate cancer.
- P504S should be used with light microscopy and potentially high molecular weight cytokeratin staining, as occasional false negatives and positives occur.
