Related Experiment Video
Updated: Aug 25, 2026

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Published on: January 22, 2014
Protein engineering 20 years on
James A Brannigan1, Anthony J Wilkinson
1Department of Chemistry, University of York, York YO10 5YW, UK. jab@ysbl.york.ac.uk
Abstract:
It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.
Related Concept Videos
What is Genetic Engineering?
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Mechanical Protein Functions
Catalytically Perfect Enzymes
Bioreactor Controls-III
Upstream Processing

