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Updated: Aug 11, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
The lineage decisions of helper T cells
Kenneth M Murphy1, Steven L Reiner
1Howard Hughes Medical Institute and Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA. murphy@pathbox.wustl.edu
Abstract:
After encountering antigen, helper T (T(H)) cells undergo differentiation to effector cells, which can secrete high levels of interferon-gamma, interleukin-4 (IL-4), IL-10 and other immunomodulators. How T(H) cells acquire, and remember, new patterns of gene expression is an area of intensive investigation. The process is remarkably plastic, with cytokines being key regulators. Extrinsic signals seem to be integrated into cell-intrinsic programming, in what is becoming an intriguing story of regulated development. We summarize the latest insights into mechanisms that govern the lineage choices that are made during T(H)-cell responses to foreign pathogens.
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