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Related Experiment Videos

Transdifferentiation--fact or artifact.

Ying Liu1, Mahendra S Rao

  • 1Laboratory of Neurosciences, National Institute on Aging, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA.

Journal of Cellular Biochemistry
|December 4, 2002
PubMed
Summary

Cell fate restriction during development can be reversed through transdifferentiation, revealing new cell potentials. This process, driven by epigenetic changes, may be controllable but likely requires complex manipulation for reliability.

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Epigenetics

Background:

  • Normal development involves progressive restriction of cell potential.
  • Recent evidence challenges the irreversibility of this developmental trajectory.
  • Novel cell phenotypes can emerge from stem, progenitor, and differentiated cells.

Purpose of the Study:

  • To explore the phenomenon of cell transdifferentiation and dedifferentiation.
  • To understand the underlying mechanisms of altered cell fate restriction.
  • To investigate the potential for controlling transdifferentiation.

Main Methods:

  • Analysis of existing evidence for transdifferentiation and dedifferentiation.
  • Examination of developmental processes and epigenetic regulation.

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  • Hypothesizing mechanisms for controlling cell fate plasticity.

    • Main Results:

    • Some observed cell fate changes are difficult to explain by contamination or fusion.
    • True transdifferentiation and dedifferentiation events are suggested by the data.
    • Epigenetic control of gene expression is proposed as a key mechanism.

    Conclusions:

    • Transdifferentiation involves the subversion of epigenetic controls.
    • Identifying factors to control transdifferentiation may be possible.
    • Transdifferentiation is predicted to be unreliable and require complex interventions.