Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular recognition in antibody-antigen complexes.

Eric J Sundberg1, Roy A Mariuzza

  • 1Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA.

Advances in Protein Chemistry
|December 5, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>N</i>‑Azidoacetylglucosamine (GlcNAz)-Derived Sugar Oxazolines as a New Class of Substrates for Chemoenzymatic Site-Specific Antibody Bioconjugation.

JACS Au·2026
Same author

Asymmetrically glycosylated IgG1 antibodies are universal and drive human disease.

Nature communications·2025
Same author

Structural insights into clonal restriction and diversity in T cell recognition of two immunodominant SARS-CoV-2 nucleocapsid epitopes.

Nature communications·2025
Same author

FcγRI is the key determinant of antibody-mediated Zika virus infection of human placental macrophages.

bioRxiv : the preprint server for biology·2025
Same author

Lipid A mimetic BECC438 potentiates durable and balanced antibody responses in an ovalbumin murine model of vaccination.

ImmunoHorizons·2025
Same author

Molecular basis of Fab-dependent IgA antibody recognition by gut-bacterial metallopeptidases.

The EMBO journal·2025

Antibody-antigen interactions reveal common structural features and plasticity crucial for immune recognition. Studies highlight thermodynamic hot spots and induced fit mechanisms in antibody binding and affinity maturation.

Area of Science:

  • Structural Biology
  • Immunology
  • Biophysics

Background:

  • Antibody-antigen interfaces are increasingly studied as models for protein-protein interactions.
  • Understanding these interactions is key to immunological function and drug development.

Purpose of the Study:

  • To describe common structural aspects of antibody-antigen interfaces.
  • To explore their roles in antibody cross-reactivity and molecular mimicry.
  • To analyze thermodynamic properties and plasticity in antigen recognition.

Main Methods:

  • Structural analysis of antibody-antigen complexes.
  • Extensive mutagenesis and thermodynamic analysis.
  • Thermodynamic mapping of interfaces and identification of hot spots.

Related Experiment Videos

Main Results:

  • Common structural features identified in antibody-antigen interfaces.
  • Thermodynamic hot spots and mechanisms for accommodating mutations were elucidated.
  • Protein plasticity, including entropic control and induced fit, plays a functional role in antigen recognition.

Conclusions:

  • Structural and thermodynamic analyses provide deep insights into antibody-antigen interactions.
  • Protein plasticity is essential for antibody affinity maturation and antigen binding specificity.