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Updated: Sep 28, 2026
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Cytologic diagnosis of estrogen and progesterone receptors in breast imprints
Masaomi Sasaki1, Ken Morimoto, Masae Koh
1Department of Pathology, Osaka City University Hospital, Second Deaprtment of Surgery, Osaka City University Medical School, Osaka, Japan.
Objective:
To investigate estrogen receptor (ER) and progesterone receptor (PR) levels in imprint specimens obtained at breast surgery and to compare their correlation with that of standard methods.
Study Design:
Imprint specimens for cytology were obtained from 101 mass-forming lesions in 66 patients, and specimens were frozen in liquid nitrogen for later assay. The imprint specimens were immunocytochemically (ICC) stained by monoclonal antibody to ER or PR; diaminobenzidine-stained cell nuclei in clusters were regarded as positive. Tissue specimens were assayed by the standard method of dextran-coated charcoal assay (DCC) and enzyme immunoassay.
Results:
Forty-five primary breast cancer lesions, 2 contralateral breast cancer, 49 dissected nodes and 5 benign breast lesions were collected. The correlation between DCC and ICC was 81% (82/101) for ER and 74% (66/101) for PR. That between EIA and ICC was 88% (88/99) for ER and 80% (79/100) for PR, higher than that between DCC and ICC for ER and PR.
Conclusion:
ICC assessment of ER or PR on imprint cytology is a promising clinical test with an acceptable correlation.
