Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An electrospray ionization source for integration with microfluidics.

Jun Kameoka1, Reid Orth, Bojan Ilic

  • 1School of Applied and Engineering Physics and Analytical Toxicology College of Veterinary Medicine, Cornell University, 927 Warren Drive, Ithaca, New York 14850, USA.

Analytical Chemistry
|December 5, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lyocell-modal thread microfluidic platform integrated with a microneedle sensor for lactate detection in saliva.

Lab on a chip·2026
Same author

Transfer, integration, and inverse design of metasurfaces in suspended membranes.

Moore and more·2026
Same author

Tunable phase-change metasurfaces coupled with mid-infrared molecular vibrations.

Nanotechnology·2025
Same author

Smart Catheters for Diagnosis, Monitoring, and Therapy.

Advanced healthcare materials·2025
Same author

Activation of <i>ankrd1a</i> expression marks newly forming myofibers and regulates muscle cell differentiation in adult zebrafish skeletal muscle repair.

American journal of physiology. Cell physiology·2025
Same author

Scenting Ketones in the Defense Glands of Two Julids From the Caucasus (Arthropoda, Myriapoda, Diplopoda, Julida).

Journal of chemical ecology·2025
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

Researchers developed a novel electrospray ionization (ESI) device using microfluidics and thin-film technology for mass spectrometry. This new ESI source enables stable Taylor cone formation and multiplexed analysis with minimal cross-contamination.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Mass Spectrometry

Background:

  • Electrospray ionization (ESI) is a crucial technique for interfacing liquid samples with mass spectrometry.
  • Traditional ESI sources can face challenges with stability and integration into microfluidic systems.
  • Developing miniaturized and robust ESI sources is essential for high-throughput and portable analytical devices.

Purpose of the Study:

  • To demonstrate a novel electrospray ionization (ESI) device integrated with a microfluidic channel and a shaped thin-film tip.
  • To investigate the performance of this new ESI source for mass spectrometric analysis.
  • To assess the feasibility of creating multichannel ESI arrays for multiplexed analysis.

Main Methods:

  • Fabrication of a microfluidic channel (20 µm wide, 10 µm deep) in a cyclo olefin substrate using lithography and etching.

Related Experiment Videos

  • Formation of a triangular-shaped thin polymer tip using lithography and etching.
  • Bonding of the tip to the microfluidic channel to create a stable Taylor cone formation site.
  • Interfacing the microfluidic ESI device with a time-of-flight mass spectrometer (TOFMS).
  • Testing with various solutions and evaluating a multichannel array system.

    • Main Results:

    • Successful demonstration of a stable Taylor cone at the tip apex, enabling electrospray ionization.
    • Reliable mass spectrometric analysis of sprayed solutions using the developed ESI source.
    • Fabrication of integrated multichannel systems with high geometrical fidelity using lithographic methods.
    • Demonstration of multiplexed analysis with no significant cross-contamination between closely spaced channels.

    Conclusions:

    • The novel microfluidic ESI device with a shaped thin-film tip provides a stable and efficient ionization source for mass spectrometry.
    • The lithographic fabrication approach allows for the easy creation of scalable, integrated multichannel ESI arrays.
    • This technology holds promise for developing advanced, high-throughput analytical systems with reduced sample consumption and improved spatial resolution.