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Electron tomography: towards visualizing the molecular organization of the cytoplasm
1Department of Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany. baumeist@biochem.mpg.de
Current Opinion in Structural Biology
|December 5, 2002
Abstract:
Electron tomography of thin sections of plastic-embedded biological material provides valuable insights into the organellar organization of cells. With the advent of automated data-acquisition procedures, it has become possible to study radiation-sensitive specimens, such as frozen-hydrated organelles and whole cells. This opens up exciting perspectives for visualizing the molecular organization of the cytoplasm.