Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reduced delayed-rectifier K+ current in the learning mutant rutabaga.

Waleed B Alshuaib1, Mini V Mathew

  • 1Department of Physiology, Faculty of Medicine, Kuwait University, Kuwait. Waleed@hsc.Kuniv.edu.Kw

Learning & Memory (Cold Spring Harbor, N.Y.)
|December 5, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrogen peroxide-induced reduction of delayed rectifier potassium current in hippocampal neurons involves oxidation of sulfhydryl groups.

Brain research·2013
Same author

Oxidative stress alters physiological and morphological neuronal properties.

Neurochemical research·2007
Same author

Modulation of neuronal [Ca2+]i by caffeine is altered with aging.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2006
Same author

Vitamins C and E modulate neuronal potassium currents.

The Journal of membrane biology·2006
Same author

Caffeine modulates potassium currents in Drosophila neurons.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2006
Same author

Transient K+ current is blocked by lanthanum in Drosophila neurons.

Neurochemical research·2005
Same journal

Transition and renewal at <i>Learning & Memory</i>.

Learning & memory (Cold Spring Harbor, N.Y.)·2026
Same journal

Experimentally induced encoding variability influences mnemonic discrimination: evidence from human behavioral data and global matching models.

Learning & memory (Cold Spring Harbor, N.Y.)·2026
Same journal

Temporal proximity to sleep determines emotional memory interference.

Learning & memory (Cold Spring Harbor, N.Y.)·2026
Same journal

Item recognition is associated with gut microbiota composition in healthy humans.

Learning & memory (Cold Spring Harbor, N.Y.)·2026
Same journal

Memory-based similar lure rejections promote subsequent memory for relative recency.

Learning & memory (Cold Spring Harbor, N.Y.)·2026
Same journal

Chronic stress enhances threat responding and impacts fear extinction.

Learning & memory (Cold Spring Harbor, N.Y.)·2026
See all related articles

The rutabaga mutant in Drosophila exhibits memory deficits and reduced cyclic adenosine monophosphate (cAMP). This study found a smaller delayed-rectifier potassium current (IK(DR)) in rutabaga neurons, suggesting altered neuronal excitability.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • The rutabaga mutant in Drosophila displays impaired short-term memory and reduced intracellular cyclic adenosine monophosphate (cAMP).
  • Understanding the molecular mechanisms underlying memory formation is crucial for neurological research.

Purpose of the Study:

  • To investigate the characteristics of the delayed-rectifier potassium current (IK(DR)) in rutabaga mutant Drosophila neurons compared to wild-type.
  • To determine if alterations in IK(DR) contribute to the memory deficits observed in the rutabaga mutant.

Main Methods:

  • Whole-cell patch-clamp technique applied to cultured Drosophila neurons from embryonic neuroblasts.
  • Measurement of IK(DR) in Ca(2+)-free solution and after application of tetraethylammonium chloride (TEA).

Related Experiment Videos

Main Results:

  • IK(DR) was significantly smaller in rutabaga neurons (368 pA) than in wild-type neurons (541 pA).
  • No significant difference in IK(DR) inactivation was observed between genotypes.
  • Application of TEA reduced IK(DR) by 46% in wild-type but only 28% in rutabaga, indicating a reduced TEA-sensitive component in rutabaga.

Conclusions:

  • The delayed-rectifier potassium current (IK(DR)) is carried by both TEA-sensitive and TEA-insensitive channels.
  • Rutabaga neurons show reduced expression of TEA-sensitive channels.
  • Altered neuronal excitability due to reduced IK(DR) may underlie the learning and memory deficits in the rutabaga mutant.